hrp conjugated goat antimouse igg1  (Bioss)

Journal: ImmunoHorizons

Article Title: Deficiency of H3K27 histone demethylase UTX in T cells blunts allergic sensitization and anaphylaxis to peanut.

doi: 10.1093/immhor/vlaf008

Figure Lengend Snippet: Figure 1. T cell-specific deficiency in UTX blunts allergic sensitization and anaphylaxis in a peanut airway sensitization allergy model. (A) Experimental Timeline for peanutþLPS airway sensitization. (B) Peanut specific IgE and (C) IgG1 at experimental day 14. (D) Core body temperatures recorded after intraperitoneal (i.p) peanut challenge in all mice, (E) Serum mouse mast cell protease (MMCP1) levels following i.p challenge. Data shown are pooled from 5 experiments, with n ¼ 46-49 mice per group. Statistical analysis: Unpaired t-test in (B), (C), and (E) comparing UTXfl/fl and UTX-TCD in each category. Two-way ANOVA In D. P < 0.05, P < 0.01, P < 0.001, P < 0.0001.

Article Snippet: Total IgG1 was detected using HRP-conjugated goat antimouse IgG1 (Southern Biotech, cat. no.1070-05) and TMB substrate, while total IgE was detected using HRP-conjugated goat anti-mouse IgE (Southern Biotech cat. no.1110-05) and TMB substrate.

Techniques:

Journal: ImmunoHorizons

Article Title: Deficiency of H3K27 histone demethylase UTX in T cells blunts allergic sensitization and anaphylaxis to peanut.

doi: 10.1093/immhor/vlaf008

Figure Lengend Snippet: Figure 3. Frequencies of Th2, but not Tfh cells, in UTX-TCD mice correlate with blunted anaphylaxis and reduced peanut-specific IgE and IgG1. Linear regression analysis comparing Tfh cell frequencies with (A) drop in core body temperature 45 min post challenge, (B) peanut-specific IgE (PNsIgE) at day 14, and (C) peanut-specific IgG1 (PNsIgG1) at day 14. (D) Linear regression analysis comparing CD4þ IL-4þ (Th2) cell frequencies with drop in core body temperature 45 min post challenge, (E) peanut-specific IgE (PNsIgE) at day 14, and (F) peanut-specific IgG1 (PNsIgG1) at day 14. UTXfl/fl control mice are shown in red, and UTX-TCD mice in blue. F-test degree of reduction values (F), R2 and P values for each linear regression analysis are displayed above each plot. Data are from one experiment and are representative of two independent experiments.

Article Snippet: Total IgG1 was detected using HRP-conjugated goat antimouse IgG1 (Southern Biotech, cat. no.1070-05) and TMB substrate, while total IgE was detected using HRP-conjugated goat anti-mouse IgE (Southern Biotech cat. no.1110-05) and TMB substrate.

Techniques: Control

Journal: ImmunoHorizons

Article Title: Deficiency of H3K27 histone demethylase UTX in T cells blunts allergic sensitization and anaphylaxis to peanut.

doi: 10.1093/immhor/vlaf008

Figure Lengend Snippet: Figure 4. Mice with UTX-deficient T cells skew toward systemic type 2 immune responses, with elevations in both effector T-helper 2 CD4þ T cells and type 2-dependent antibody isotypes, independent of allergic sensitization. (A) Representative flow cytometry plots showing splenic CD44þIL-4þ of CD4þTCRβþ Th2 cells. (B) Summary plots showing frequency, absolute number, and mean fluorescence intensity (MFI) of CD4þCD44þ IL-4þ Th2 cells. (C) Representative flow cytometry plots showing splenic CD44þIL-13þ of CD4þTCRβþ Th2 cells. (D) Summary plots showing frequency, absolute number, and mean fluorescence intensity (MFI) of CD4þCD44þ IL-13þ Th2 cells. (E) Representative flow cytometry showing CD4 and GATA3 staining of gated CD3þ T cells in peripheral blood. (F) Summary plots showing frequency, absolute number, and MFI of CD3þCD4þGATA3þ Th2 cells. (G) Total IgE (n ¼ 62 mice), IgG1 (n ¼ 37-42 mice), and IgG2c (n ¼ 29 mice) at baseline in UTXfl/fl and UTX-TCD mice. Data shown in B and D, except MFI, are pooled from 2 independent experiments, n ¼ 8-9 mice per group. MFI values in B and D and data in E and F are representative of 2 independent experiments with n ¼ 5 mice per group. Statistical analysis: Unpaired t-test in B, D, F, and G, comparing UTXfl/fl and UTX-TCD. P < 0.05, P < 0.01, ns, not significant; Th2, T helper 2.

Article Snippet: Total IgG1 was detected using HRP-conjugated goat antimouse IgG1 (Southern Biotech, cat. no.1070-05) and TMB substrate, while total IgE was detected using HRP-conjugated goat anti-mouse IgE (Southern Biotech cat. no.1110-05) and TMB substrate.

Techniques: Flow Cytometry, Fluorescence, Staining

Journal: Bioengineering & translational medicine

Article Title: Intra-lymph node crosslinking of antigen-bearing polymers enhances humoral immunity and dendritic cell activation.

doi: 10.1002/btm2.10705

Figure Lengend Snippet: FIGURE 5 Immunological characterization of the intra-LN crosslinking platform. (a) Legend applies to all panels in this figure. (b) Anti-OVA IgG titers measured over 14 weeks for all groups, n = 6. PEG–OVA + “Click” is statistically greater than PEG–OVA, PEG + Free OVA, and Free OVA at week 14. Alum is statistically greater than all other groups at week 14. Error bars represent SEM. (c) Anti-OVA IgG subclasses measured for the two crosslinking groups, n = 6. PEG–OVA, PEG + Free OVA, and Free OVA are not presented as all were below the limit of detection. (d–j) Flow cytometry of cells from targeted lymph nodes, n = 5. (d) Representative plots of non-B/T lymphocytes expressing the activation markers CD80 and CD40. A graphical representation of each of these markers is shown in (e) and (f). (g) Expression of MHC I presenting the OVA peptide SIINFEKL in non-B/T lymphocytes. (h) Percent of non-B/T cells expressing the dendritic cell marker CD11c. (i) Percent of non-B/T cells co-expressing CD11c and CD40. (j) Percent of non-B/T cells co-expressing CD11c and CD80.

Article Snippet: HRP-conjugated goat antimouse IgG1, IgG2a, IgG2b, and IgG3 antibodies (Jackson ImmunoResearch) were each substituted for anti-mouse IgG and diluted 1:1000 in blocking solution.

Techniques: Flow Cytometry, Expressing, Activation Assay, Marker

Journal: Biomaterials

Article Title: Self-assembled protein vesicles as vaccine delivery platform to enhance antigen-specific immune responses.

doi: 10.1016/j.biomaterials.2024.122666

Figure Lengend Snippet: Fig. 5. ELISA endpoint titers of OVA specific IgG1 (a) and IgG2a (b) antibodies after prime and boost immunization. Each mouse was immunized with 10 μg OVA-ZE in both soluble OVA-ZE group and OVA protein vesicle group. Titer values too low for detection were arbitrarily set at 10. (**p < 0.01).

Article Snippet: After serum incubation, plates were washed with PBST and incubated with HRP-conjugated goat antimouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat antimouse IgG2a-HRP antibodies (Southern Biotech) at a 1:4000 dilution in 0.1 % BSA/PBST for 1 h at room temperature followed by washing with PBST.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Biomaterials

Article Title: Self-assembled protein vesicles as vaccine delivery platform to enhance antigen-specific immune responses.

doi: 10.1016/j.biomaterials.2024.122666

Figure Lengend Snippet: Fig. 6. Comparison of ELISA endpoint titers of IgG1 (a) and IgG2a (b) against OVA antigen and pZR-ELP antigen. Titer values too low for detection were arbitrarily set at 10. Data labeled “Against OVA” in red is replotted from Fig. 5 to compare with the titers against pZR-ELP. (**p < 0.01, *p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: After serum incubation, plates were washed with PBST and incubated with HRP-conjugated goat antimouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat antimouse IgG2a-HRP antibodies (Southern Biotech) at a 1:4000 dilution in 0.1 % BSA/PBST for 1 h at room temperature followed by washing with PBST.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Labeling